Chromosome-Level Genome Assembly of Cerasus humilis Using PacBio and Hi-C Technologies
نویسندگان
چکیده
منابع مشابه
De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds.
The Zika outbreak, spread by the Aedes aegypti mosquito, highlights the need to create high-quality assemblies of large genomes in a rapid and cost-effective way. Here we combine Hi-C data with existing draft assemblies to generate chromosome-length scaffolds. We validate this method by assembling a human genome, de novo, from short reads alone (67× coverage). We then combine our method with dr...
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The three-dimensional conformation of chromosomes in the nucleus is important for many cellular processes, including the regulation of gene expression, DNA replication, and chromatin structure [1]. Despite having the entire sequence of the genome, very little has been understood about three-dimensional chromosome conformation beyond the scale of the nucleosome. However, recent advances in molec...
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Plasmodium knowlesi has risen in importance as a zoonotic parasite that has been causing regular episodes of malaria throughout South East Asia. The P. knowlesi genome sequence generated in 2008 highlighted and confirmed many similarities and differences in Plasmodium species, including a global view of several multigene families, such as the large SICAvar multigene family encoding the variant ...
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Most genome browsers display DNA linearly, using single-dimensional depictions that are useful to examine certain epigenetic mechanisms such as DNA methylation. However, these representations are insufficient to visualize intrachromosomal interactions and relationships between distal genome features. Relationships between DNA regions may be difficult to decipher or missed entirely if those regi...
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MOTIVATION PacBio sequencers produce two types of characteristic reads (continuous long reads: long and high error rate and circular consensus sequencing: short and low error rate), both of which could be useful for de novo assembly of genomes. Currently, there is no available simulator that targets the specific generation of PacBio libraries. RESULTS Our analysis of 13 PacBio datasets showed...
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ژورنال
عنوان ژورنال: Frontiers in Genetics
سال: 2020
ISSN: 1664-8021
DOI: 10.3389/fgene.2020.00956